Difference between revisions of "Part:BBa K1679019:Design"

 
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===Design Notes===
 
===Design Notes===
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There is a RFP reproter device downstream.It has a RNA Thermometers(RNAT) which are temperature-sensing RNA sequences in 5’UTR of their mRNAs. At low temperature, RNAT folds into structure, blocking access of ribosome; at high temperature, RNAT switch from off to open conformation, increasing the efficiency of translation initiation.When the temperature rises above 37°C, RFP would be expressed in theory.
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There is a GFP reporter device except promoter in front of the RFP reproter device.
  
 
===Source===
 
===Source===

Latest revision as of 22:48, 18 September 2015

GFP+Promoter+RNA thermometer (FourU) +mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 890
    Illegal NheI site found at 913
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1539
    Illegal AgeI site found at 1651
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


Design Notes

There is a RFP reproter device downstream.It has a RNA Thermometers(RNAT) which are temperature-sensing RNA sequences in 5’UTR of their mRNAs. At low temperature, RNAT folds into structure, blocking access of ribosome; at high temperature, RNAT switch from off to open conformation, increasing the efficiency of translation initiation.When the temperature rises above 37°C, RFP would be expressed in theory.

There is a GFP reporter device except promoter in front of the RFP reproter device.

Source

BBa_I13504 BBa_J23119 BBa_K115002 BBa_E1010


References