Difference between revisions of "Part:BBa K1813016"

Latest revision as of 22:48, 18 September 2015

nicE Expression Casette

The enzyme coded for by nicE is a maleate isomerase that converts Maleic Acid to Fumaric Acid.

Sequence and Features

Assembly Compatibility:

This is a composite part for BBa_K1813022.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1813016 short</partinfo>
-ptac nicE term
+The enzyme coded for by <i>nicE</i> is a maleate isomerase that converts Maleic Acid to Fumaric Acid.
 <!-- Add more about the biology of this part here
@@ Line 12: / Line 11: @@
 <partinfo>BBa_K1813016 SequenceAndFeatures</partinfo>
+<h2>Background of <i>nicE</i></h2>
-<!-- Uncomment this to enable Functional Parameter display
-<h2>Background of <i>Ptac NicE Term</i></h2>
-<h4>Part Description:</h4>
+<html>
-<p>
+<h4>Part Function:</h4>
-</p>
+<p>This is a composite part for <a href="https://parts.igem.org/Part:BBa_K1813022">BBa_K1813022</a>. </p>
+<!-- -->
-<h4>Part Origin</h4>
+</html>
-<p>where part originated and <i>Genus species</i> more words if needed</p>
-<h4>Part Function</h4>
-<p>part functionstuff </p>
-<h4>Design and Acquisition</h4>
-<p>
-After synthesizing a codon-optimized NicE, we used standard assembly to created a composite part composed of NicE driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicE expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicE. All nicE constructs are contained within standard pSB1C3 vectors.</p>
-<h4>Experience</h4>
-<p><p>