Difference between revisions of "Part:BBa K1602002"
 
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         <h1>Aconitate hydratase - acnA</h1>
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         <h1>B0034-yjhG</h1>
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        This composite represents the <small>D</small>-xylonic acid dehydratases <i>yjhG</i> plus the ribosomal binding site B0034.
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===<h2>Usage</h2>===
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        This part is a composite of one coding gene and a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>).
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        This part makes it possible to establish an ethyleneglycol producing pathway in other hosts than <i>E. coli</i> if its combined with other enzymes of the pathway <a href="https://parts.igem.org/Part:BBa_K1602017">(BBa_K1602017)</a> <a href="https://parts.igem.org/Part:BBa_K1602014">(BBa_K1602014)</a> <a href="https://parts.igem.org/Part:BBa_K1602015">(BBa_K1602015)</a>. (1)
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                <b>acnA</b> is a gene, coding for a aconitate hydratase in <i>E.coli</i>. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.
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                    <ul>
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<!--<br>
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                        <li>yjhG</li>
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                FUELLTEXT
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                        <li>RBS-B0034</li>
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                     <!--<div style="float: right;">-->
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                    <img style="width: 300px; height: 300px; align: center; margin-left: 100px; margin-right:100px" alt="" src="https://static.igem.org/mediawiki/2015/9/9a/Citrate_synthase_gltA.png"> </div>  
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                        <li><a href="https://parts.igem.org/Part:BBa_K1602012">BBa_K1602012</a></li>
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                        <li><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></li>
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<div style="float: right;"><img style="width: 400px; height: 120px;" alt="" src="
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                    </ul>
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https://static.igem.org/mediawiki/2015/a/a1/Itaconic_acid_gene_operon.png"> </div>  
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                     <!-- BLANK -->
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                     <div align="center" valign="middle"><img class="transparent shrinkToFit" alt="https://static.igem.org/mediawiki/parts/e/e5/TU_Darmstadt_plasmid-card_B0034-yjhG.png" src="https://static.igem.org/mediawiki/parts/e/e5/TU_Darmstadt_plasmid-card_B0034-yjhG.png" height="227" width="255"></div>
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                    <b>Figure 1</b> Citrate synthase (coded by gltA); represented in oligo state as a homo-hexameric protein unit
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===<h2>Characteristics</h2>===
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===<h2>Sequence and Features</h2>===
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<partinfo>BBa_K1602002 SequenceAndFeatures</partinfo>
  
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===References===
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===<h2>Sequence and Features</h2>===
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1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.
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<partinfo>BBa_K1602001 SequenceAndFeatures</partinfo>
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Latest revision as of 22:34, 18 September 2015

B0034-yjhG

This composite represents the D-xylonic acid dehydratases yjhG plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

This part makes it possible to establish an ethyleneglycol producing pathway in other hosts than E. coli if its combined with other enzymes of the pathway (BBa_K1602017) (BBa_K1602014) (BBa_K1602015). (1)

  • yjhG
  • RBS-B0034
https://static.igem.org/mediawiki/parts/e/e5/TU_Darmstadt_plasmid-card_B0034-yjhG.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1016
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1921
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 179


References

1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.