Difference between revisions of "Part:BBa K1763439"

Revision as of 22:31, 18 September 2015

MaSp1/2[2:1]-12(T7)

This composite part consists of the MaSp1/2[2:1]-12 construct (BBa_K1763438) sublconed with a T7 RNA polymerase promoter and a strong RBS (BBa_K525998). This construct is intended to generate the MaSp1/2[2:1]-12 construct in high quantities in E. coli with an inducible T7 RNA polymerase system.

Biology

The T7 RNA polymerase expression system has been developed for large scale protein expression in E. coli. Strains carry an inducible T7 RNA polymerase gene incorporated into their genome. This allows very specific induction of protein expression because the T7 RNA polymerase promoter is not found in E. coli.

A common laboratory strain for protein expression is BL21(DE3), which is what the 2015 UCLA iGEM team used in this project. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1302
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1763439 short</partinfo>
-MaSp1 and MaSp2 12-mer block co-polymer under control of T7 promoter and RBS. Ratio of 2 MaSp1 to 1 MaSp2.
+This composite part consists of the MaSp1/2[2:1]-12 construct ([https://parts.igem.org/Part:BBa_K1763438 BBa_K1763438]) sublconed with a T7 RNA polymerase promoter and a strong RBS ([https://parts.igem.org/Part:BBa_K525998 BBa_K525998]). This construct is intended to generate the MaSp1/2[2:1]-12 construct in high quantities in E. coli with an inducible T7 RNA polymerase system.
+===Biology===
+The T7 RNA polymerase expression system has been developed for large scale protein expression in E. coli. Strains carry an inducible T7 RNA polymerase gene incorporated into their genome. This allows very specific induction of protein expression because the T7 RNA polymerase promoter is not found in E. coli.
+A common laboratory strain for protein expression is BL21(DE3), which is what the 2015 UCLA iGEM team used in this project.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===