Difference between revisions of "Part:BBa K1807002"
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<partinfo>BBa_K1807002 short</partinfo> | <partinfo>BBa_K1807002 short</partinfo> | ||
− | This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from ''Escherichia coli'' | + | This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from ''Escherichia coli''. |
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below. | ||
<html> | <html> |
Revision as of 22:27, 18 September 2015
Escherichia coli Polyphosphate Kinase Enzyme
This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli.
Usage and Biology
PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.
![](https://static.igem.org/mediawiki/2015/d/d4/PPK_activity_York.jpeg)
Akiyama, Masahiro, E. Crooke, and Arthur Kornberg. "The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein." Journal of Biological Chemistry 267.31 (1992): 22556-22561.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 396
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1750
- 1000COMPATIBLE WITH RFC[1000]