Difference between revisions of "Part:BBa E0022"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_E0022 short</partinfo>
 
<partinfo>BBa_E0022 short</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
  
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This part can be used to test promoter strength or the proper functioning of a regulator device.  The LVA tail is particularly useful when testing a regulatory device, for example to assess the efficiency of repressor.
 
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In this experiment, the E0022 part was associated with a cI regulated promoter (part R0051), resulting in K1707028.
 
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'''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170728'''
 
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[[File:Paris_Saclay_ K1707028_w1POL.png|300px]]
 
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Differential interference contrast
 
  
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[[File:Paris_Saclay_K1707028_w2Lumencor CFP 434-17.png|300px]]
 
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Excitation with led 437 nm/17nm (CFP), 100 ms of exposure
 
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:11, 18 September 2015

enhanced cyan fluorescent protein derived from A. victoria GFP

Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]