Difference between revisions of "Part:BBa K1813009"

Revision as of 22:06, 18 September 2015

nicF

nicF Gen Bank accession number Q88FY5.1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 439
1000
COMPATIBLE WITH RFC[1000]

Background of nicF:

Part Description:

Codon optimized coding sequence of nicF, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813017 and BBa_K1813023 .

Part Origin:

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function:

The enzyme coded for by nicF converts Maleamic Acid to Maleic Acid.

References:

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

@@ Line 10: / Line 10: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1813009 SequenceAndFeatures</partinfo>
 <partinfo>BBa_K1813009 parameters</partinfo>
@@ Line 18: / Line 16: @@
 <h2>Background of <i>nicF</i>:</h2>
 <h4>Part Description:</h4>
-<p>Codon optimized coding sequence of <i>nicF</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813023">BBa_K1813023</a>. </p>
+<p>Codon optimized coding sequence of <i>nicF</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813017">BBa_K1813017</a> </html> and
+<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813023">BBa_K1813023</a> </html>.
 <h4>Part Origin:</h4>