Difference between revisions of "Part:BBa K1758311:Design"
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<partinfo>BBa_K1465102 short</partinfo>
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<partinfo>BBa_K1758311 short</partinfo>
  
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<partinfo>BBa_K1465102 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1758311 SequenceAndFeatures</partinfo>
  
  
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===Design Notes===
 
  
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===Design Notes===
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We used the codon optimized, synthesized Sequence of RFP under the control of chrP  from iGEM Team BIT 2013(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K346002 " target="_blank"> BBa_K346002 </a>). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of chrP with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of chrP and the pSB1C3:
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank"><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank">cm_fwd</a> (CGGCATCAGCACCTTGTC) </p>
  
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===Source
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_chrP_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_chrP_rev" target="_blank">pSB1C3_chrP_rev</a> (CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT)</p>
  
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank">cm_rev</a> (TATACGCAAGGCGACAAG) </p>
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The original source of our chromate responsive promoter is <i>Ochrobactrum tritici</i> 5bvl1. We used a codon optimized version which we got synthesised by IDT.
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</html>
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===References===
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> pSB1C3_RFP_rev</a> (CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA) </p>
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===Source===
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Synthesized, codon optimized gene of  <i>Ochrobactrum tritici</i>
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synthesized by IDT
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</html>
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===References===  
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Synthesized by IDT
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<p>http://www.ncbi.nlm.nih.gov/nuccore/134290393 </p>
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</html>

Revision as of 22:06, 18 September 2015

_NOTOC__ mRFP under control of the chromium responsive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the codon optimized, synthesized Sequence of RFP under the control of chrP from iGEM Team BIT 2013( BBa_K346002 ). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of chrP with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of chrP and the pSB1C3:

cm_fwd (CGGCATCAGCACCTTGTC)

pSB1C3_chrP_rev (CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT)

cm_rev (TATACGCAAGGCGACAAG)

pSB1C3_RFP_rev (CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA)

Source

Synthesized, codon optimized gene of Ochrobactrum tritici synthesized by IDT

References

Synthesized by IDT

http://www.ncbi.nlm.nih.gov/nuccore/134290393