Difference between revisions of "Part:BBa K1813008"
Line 17: Line 17:
 
<h2>Background of <i>nicE</i>:</h2>
 
<h2>Background of <i>nicE</i>:</h2>
 
<h4>Part Description:</h4>
 
<h4>Part Description:</h4>
−
<p>Codon optimized coding sequence of <i>nicE</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813022">BBa_K1813022</a>. </p>
+
<p>Codon optimized coding sequence of <i>nicC</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813016">BBa_K1813016</a> </html> and
 +
<html><a href="https://parts.igem.org/Part:BBa_K1813022">BBa_K1813022</a> </html>.
 +
 
  
 
<h4>Part Origin:</h4>
 
<h4>Part Origin:</h4>
Line 23: Line 25:
  
 
<h4>Part Function:</h4>
 
<h4>Part Function:</h4>
−
<p>The enzyme coded for by <i>nicE</i> converts Maleic Acid to Fumaric Acid.</p>
+
<p>The enzyme coded for by <i>nicE</i> is a maleate isomerase that converts Maleic Acid to Fumaric Acid.</p>
  
 
<h4>References:</h4>
 
<h4>References:</h4>
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

Revision as of 22:02, 18 September 2015

nicE

nicE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55


Background of nicE:

Part Description:

Codon optimized coding sequence of nicC, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813016 and BBa_K1813022 .


Part Origin:

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function:

The enzyme coded for by nicE is a maleate isomerase that converts Maleic Acid to Fumaric Acid.

References:

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105