Difference between revisions of "Part:BBa K1796013"

 
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<partinfo>BBa_K1796013 short</partinfo>
 
<partinfo>BBa_K1796013 short</partinfo>
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<partinfo>BBa_K1796013 parameters</partinfo>
 
<partinfo>BBa_K1796013 parameters</partinfo>
 
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==Parameter of Protein==
 +
<p> Number of amino acids: 261 </p>
 +
<p> </p>
 +
<p> Molecular weight: 28332.5 </p>
 +
<p> </p>
 +
<p> Theoretical pI: 5.51 </p>
 +
<p> </p>
 +
<p> Amino acid composition:  </p>
 +
<p> </p>
 +
<p> Ala (A)  26      10.0% </p>
 +
<p> </p>
 +
<p> Arg (R)  18      6.9% </p>
 +
<p> </p>
 +
<p> Asn (N)  7      2.7% </p>
 +
<p> </p>
 +
<p> Asp (D)  12      4.6% </p>
 +
<p> </p>
 +
<p> Cys (C)  8      3.1% </p>
 +
<p> </p>
 +
<p> Gln (Q)  7      2.7% </p>
 +
<p> </p>
 +
<p> Glu (E)  20      7.7% </p>
 +
<p> </p>
 +
<p> Gly (G)  31      11.9% </p>
 +
<p> </p>
 +
<p> His (H)  8      3.1% </p>
 +
<p> </p>
 +
<p> Ile (I)  13      5.0% </p>
 +
<p> </p>
 +
<p> Leu (L)  27      10.3% </p>
 +
<p> </p>
 +
<p> Lys (K)  6      2.3% </p>
 +
<p> </p>
 +
<p> Met (M)  13      5.0% </p>
 +
<p> </p>
 +
<p> Phe (F)  3      1.1% </p>
 +
<p> </p>
 +
<p> Pro (P)  12      4.6% </p>
 +
<p> </p>
 +
<p> Ser (S)  10      3.8% </p>
 +
<p> </p>
 +
<p> Thr (T)  12      4.6% </p>
 +
<p> </p>
 +
<p> Trp (W)  3      1.1% </p>
 +
<p> </p>
 +
<p> Tyr (Y)  9      3.4% </p>
 +
<p> </p>
 +
<p> Val (V)  16      6.1% </p>
 +
<p> </p>
 +
<p> Pyl (O)  0      0.0% </p>
 +
<p> </p>
 +
<p> Sec (U)  0      0.0% </p>
 +
<p> </p>
 +
<p> (B)  0          0.0% </p>
 +
<p> </p>
 +
<p> (Z)  0          0.0% </p>
 +
<p> </p>
 +
<p> (X)  0          0.0% </p>
 +
<p> </p>
 +
<p> Total number of negatively charged residues (Asp + Glu): 32 </p>
 +
<p> Total number of positively charged residues (Arg + Lys): 24 </p>
 +
<p> </p>
 +
<p> Atomic composition:Carbon      C              1231 </p>
 +
<p> Hydrogen    H        1964 </p>
 +
<p> Nitrogen    N          354 </p>
 +
<p> Oxygen      O          371 </p>
 +
<p> Sulfur      S          21 </p>
 +
<p> </p>
 +
<p> Formula: C1231H1964N354O371S21Total number of atoms: 3941 </p>
 +
<p> </p>
 +
<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 +
<p> </p>
 +
<p> Ext. coefficient    30410 </p>
 +
<p> Abs 0.1% (=1 g/l)  1.073, assuming all pairs of Cys residues form cystines </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Ext. coefficient    29910 </p>
 +
<p> Abs 0.1% (=1 g/l)  1.056, assuming all Cys residues are reduced </p>
 +
<p> </p>
 +
<p> Estimated half-life:The N-terminal of the sequence considered is A (Ala). </p>
 +
<p> </p>
 +
<p> The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro). </p>
 +
<p>                             >20 hours (yeast, in vivo). </p>
 +
<p>                             >10 hours (Escherichia coli, in vivo). </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Instability index:The instability index (II) is computed to be 43.97 </p>
 +
<p> This classifies the protein as unstable. </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Aliphatic index: 87.51 </p>
 +
<p> </p>
 +
<p> Grand average of hydropathicity (GRAVY): -0.097 </p>

Revision as of 21:40, 18 September 2015

hesA promoted from Paenibacillus sp. WLY78

Function has not so far been determined , but Deletion analysis indicates that in addition to the core nif genes, hesA plays an important role in nitrogen fixation and is responsive to the availability of molybdenum. HesA a member of the ThiF-MoeB-HesA family and contains an N-terminal nucleotide binding domain and a C-terminal MoeZ/MoeB-like domain. The ThiF-MoeB-HesA family engage in an ATP-dependent process that activates the C-terminus of partner ubiquitin-like proteins by forming an acyl adenylate complex that facilitates sulfur transfer.

First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 143
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 143
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 143
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 261

Molecular weight: 28332.5

Theoretical pI: 5.51

Amino acid composition: 

Ala (A) 26 10.0%

Arg (R) 18 6.9%

Asn (N) 7 2.7%

Asp (D) 12 4.6%

Cys (C) 8 3.1%

Gln (Q) 7 2.7%

Glu (E) 20 7.7%

Gly (G) 31 11.9%

His (H) 8 3.1%

Ile (I) 13 5.0%

Leu (L) 27 10.3%

Lys (K) 6 2.3%

Met (M) 13 5.0%

Phe (F) 3 1.1%

Pro (P) 12 4.6%

Ser (S) 10 3.8%

Thr (T) 12 4.6%

Trp (W) 3 1.1%

Tyr (Y) 9 3.4%

Val (V) 16 6.1%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 32

Total number of positively charged residues (Arg + Lys): 24

Atomic composition:Carbon C 1231

Hydrogen H 1964

Nitrogen N 354

Oxygen O 371

Sulfur S 21

Formula: C1231H1964N354O371S21Total number of atoms: 3941

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 30410

Abs 0.1% (=1 g/l) 1.073, assuming all pairs of Cys residues form cystines

Ext. coefficient 29910

Abs 0.1% (=1 g/l) 1.056, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 43.97

This classifies the protein as unstable.

Aliphatic index: 87.51

Grand average of hydropathicity (GRAVY): -0.097