Difference between revisions of "Part:BBa K1602013"
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+ | <h1>Aldehyde reductase - YqhD</h1> | ||
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+ | <i>YqhD</i> encodes for a NADP<sup>+</sup> dependent aldehyde reductase from <i>E. coli</i>. | ||
+ | The enzyme is able to reduce a broad range of small aldehyde substrates like glycoaldehyde to ethyleneglycol. | ||
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+ | <img class="shrinkToFit transparent" alt="https://static.igem.org/mediawiki/parts/8/81/BBa_K1602013_image_1.png" src="https://static.igem.org/mediawiki/parts/8/81/BBa_K1602013_image_1.png" height="407" width="213"> | ||
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− | + | ===<h2>Characteristics</h2>=== | |
− | < | + | |
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+ | <table> | ||
+ | <colgroup width=50% span="4"> | ||
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+ | Molecular Weight | ||
+ | </td> | ||
+ | <td> | ||
+ | 42097.02 | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | Residues | ||
+ | </td> | ||
+ | <td> | ||
+ | 387 | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | Charge | ||
+ | </td> | ||
+ | <td> | ||
+ | -3.5 | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | Isoelectric Point | ||
+ | </td> | ||
+ | <td> | ||
+ | 6.0466 | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | A280 Molar Extinction Coefficients | ||
+ | </td> | ||
+ | <td> | ||
+ | 42400 (reduced) no intramolecular cystine bridges possible | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | [Data taken from PEPSTATS] | ||
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+ | </tr> | ||
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+ | </table> | ||
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+ | </body> | ||
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− | + | ===<h2>Sequence and Features</h2>=== | |
− | === | + | |
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<partinfo>BBa_K1602013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1602013 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 21:34, 18 September 2015
Aldehyde reductase - YqhD
YqhD encodes for a NADP+ dependent aldehyde reductase from E. coli. The enzyme is able to reduce a broad range of small aldehyde substrates like glycoaldehyde to ethyleneglycol. | |
Characteristics
Molecular Weight | 42097.02 |
Residues | 387 |
Charge | -3.5 |
Isoelectric Point | 6.0466 |
A280 Molar Extinction Coefficients | 42400 (reduced) no intramolecular cystine bridges possible |
[Data taken from PEPSTATS] |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 424
Illegal AgeI site found at 618 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1081