Difference between revisions of "Part:BBa K1602003"
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| − | <h1> | + | <h1>Cis-aconitate decarboxylase (cadA) from Aspergillus terreus</h1> |
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| − | <b>cadA</b> is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The | + | <b>cadA</b> is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion. |
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<source src="https://static.igem.org/mediawiki/2015/2/2d/CadA_low_hq.mp4" type="video/mp4"> | <source src="https://static.igem.org/mediawiki/2015/2/2d/CadA_low_hq.mp4" type="video/mp4"> | ||
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<b>Figure 1</b> cis-aconitate decarboxylase (coded by cadA) | <b>Figure 1</b> cis-aconitate decarboxylase (coded by cadA) | ||
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| + | Improbability of expression in inclusion bodies | ||
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| + | 0.607 | ||
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[Data taken from PEPSTATS] | [Data taken from PEPSTATS] | ||
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===<h2>Sequence and Features</h2>=== | ===<h2>Sequence and Features</h2>=== | ||
<partinfo>BBa_K1602003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1602003 SequenceAndFeatures</partinfo> | ||
Latest revision as of 21:30, 18 September 2015
Cis-aconitate decarboxylase (cadA) from Aspergillus terreus
| cadA is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion. | |
| Figure 1 cis-aconitate decarboxylase (coded by cadA) |
Characteristics
| Molecular Weight | 52754.05 |
| Residues | 490 |
| Charge | -10.0 |
| Isoelectric Point | 5.1050 |
| A280 Molar Extinction Coefficients | 59360 (reduced) 60110 (cystine bridges) |
| Improbability of expression in inclusion bodies | 0.607 |
| [Data taken from PEPSTATS] |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]