Difference between revisions of "Part:BBa K1602003"

 
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         <h1>cis-Aconitate decarboxylase - cadA</h1>
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         <h1>Cis-aconitate decarboxylase (cadA) from Aspergillus terreus</h1>
 
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                 <b>cadA</b> is a gene, coding for a cis-aconitate decarboxylase in <i>Aspergillus terreus</i>. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.
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                 <b>cadA</b> is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion.
 
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                     <b>Figure 1</b> Citrate synthase (coded by gltA); represented in oligo state as a homo-hexameric protein unit
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                     <b>Figure 1</b> cis-aconitate decarboxylase (coded by cadA)
 
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            <tr>
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                <td>
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                    Molecular Weight
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                </td>
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                <td>
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                    52754.05
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                </td>
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            <tr>
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                <td>
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                    Residues
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                </td>
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                <td>
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                    490
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                    Charge
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                </td>
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                <td>
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                    -10.0
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                </td>
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            <tr>
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                <td>
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                    Isoelectric Point
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                </td>
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                <td>
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                    5.1050
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                </td>
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            </tr>
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            <tr>
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                <td>
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                    A280 Molar Extinction Coefficients
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                </td>
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                <td>
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                    59360 (reduced)  60110 (cystine bridges)
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                </td>
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            </tr>
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            <tr>
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                <td>
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                    Improbability of expression in inclusion bodies
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                </td>
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<td>
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0.607
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            <tr>
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                <td>
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                    [Data taken from PEPSTATS]
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===<h2>Sequence and Features</h2>===
 
===<h2>Sequence and Features</h2>===
<partinfo>BBa_K1602001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1602003 SequenceAndFeatures</partinfo>

Latest revision as of 21:30, 18 September 2015

Cis-aconitate decarboxylase (cadA) from Aspergillus terreus

cadA is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion.
Figure 1 cis-aconitate decarboxylase (coded by cadA)

Characteristics


Molecular Weight 52754.05
Residues 490
Charge -10.0
Isoelectric Point 5.1050
A280 Molar Extinction Coefficients 59360 (reduced) 60110 (cystine bridges)
Improbability of expression in inclusion bodies 0.607
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]