Difference between revisions of "Part:BBa K1602009"

 
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         <h1>cis-Aconitate decarboxylase - cadA</h1>
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         <h1><small>D</small>-Xylose dehydrogenase - xylB</h1>
 
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                 <b>cadA</b> is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The dehydrogenase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host.
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                 <i>xylB</i> encodes for a NAD<sup>+</sup> <small>D</small>-xylose dehydrogenase from <i>Caulobacter crescentus</i>. It
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                 catalyzes the reaction of <small>D</small>-xylose to <small>D</small>-xylonolactone. The sequence
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                 is codon optimized for the usage in <i>E. coli</i>.
                    <img style="width: 300px; height: 300px; align: center; margin-left: 100px; margin-right:100px" alt="" src="img/citrate_synthase_gltA.png">       
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                    <b>Figure 1</b> cis-aconitate decarboxylase (coded by cadA)
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                     52754.05
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                     26641.48
 
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                     490
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                     248
 
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                     -10.0
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                     -4.0
 
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                     5.1050
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                     5.1521
 
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                     59360 (reduced)  60110 (cystine bridges)
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                     36440 (reduced)  36690 (cystine bridges)
 
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===<h2>Sequence and Features</h2>===
 
===<h2>Sequence and Features</h2>===
<partinfo>BBa_K1602001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1602009 SequenceAndFeatures</partinfo>

Latest revision as of 21:29, 18 September 2015

D-Xylose dehydrogenase - xylB

xylB encodes for a NAD+ D-xylose dehydrogenase from Caulobacter crescentus. It catalyzes the reaction of D-xylose to D-xylonolactone. The sequence is codon optimized for the usage in E. coli.
https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png

Characteristics


Molecular Weight 26641.48
Residues 248
Charge -4.0
Isoelectric Point 5.1521
A280 Molar Extinction Coefficients 36440 (reduced) 36690 (cystine bridges)
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]