Difference between revisions of "Part:BBa K1587008"

 
 
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<partinfo>BBa_K1587008 short</partinfo>
 
<partinfo>BBa_K1587008 short</partinfo>
  
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Strong promoter BBa_J23119. Chimeric Red light receptor cph8 BBa_I15010 (cph1-envZ) and proteins involved in phycobilin biosynthesis ho1 (BBa_I15008), pcyA (BBa_I15009).
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Strong promoter [https://parts.igem.org/Part:BBa_J23119 BBa_J23119].Strong RBS BBa_B0030 Chimeric Red light receptor Cph8 [https://parts.igem.org/Part:BBa_I15010 BBa_I15010].
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<center>[[File:TLSE Circ3.jpg]]</center>
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<center>'''Figure 1: Chimeric Red light receptor Cph8  genetic construction'''</center>
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<center>[[File:TLSE CircSchem.jpg]]</center>
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<center>'''Figure 2: Chimeric Red light receptor Cph8 (Cph1-EnvZ) is inactivated by phycobilin PCB photosynthetic light-harvesting complexes preventing the phosphorylation of OmpR'''</center>
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Phosphorylation of OmpR by Cph8 inhibits the expression of the genes regulated by promoter P<sub>OmpC</sub>. Since OmpR and P<sub>OmpC</sub> are naturaly found in ''E.coli'', this biobrick enables a circadian expression of genes regulated by P<sub>OmpC</sub> in E. coli strain deleted for EnvZ.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 21:09, 18 September 2015

Red light biosensor

Strong promoter BBa_J23119.Strong RBS BBa_B0030 Chimeric Red light receptor Cph8 BBa_I15010.

TLSE Circ3.jpg
Figure 1: Chimeric Red light receptor Cph8 genetic construction


TLSE CircSchem.jpg
Figure 2: Chimeric Red light receptor Cph8 (Cph1-EnvZ) is inactivated by phycobilin PCB photosynthetic light-harvesting complexes preventing the phosphorylation of OmpR

Phosphorylation of OmpR by Cph8 inhibits the expression of the genes regulated by promoter POmpC. Since OmpR and POmpC are naturaly found in E.coli, this biobrick enables a circadian expression of genes regulated by POmpC in E. coli strain deleted for EnvZ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 414
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]