Difference between revisions of "Part:BBa K1766006"

Latest revision as of 21:05, 18 September 2015

MicF regulated LuxI

LuxI is a quorum synthase which produces 3-oxo-hexanoyl homoserine lactones (OHHL). In this construct LuxI has been fused to the ompF 5’UTR. This is the target for micF regulatory RNA. In wild type E. coli micF will inhibit translation of LuxI at high osmolarity conditions. We therefore expect MicF-Regulated-LuxI to produce more OHHL at low osmolarity conditions than at high osmolarity.

Due to time constraints iGEM Stockholm 2015 were unable to construct this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 839
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1766006 short</partinfo>
-long description
+LuxI is a quorum synthase which produces 3-oxo-hexanoyl homoserine lactones (OHHL). In this construct LuxI has been fused to the ompF 5’UTR. This is the target for micF regulatory RNA. In wild type ''E. coli'' micF will inhibit translation of LuxI at high osmolarity conditions. We therefore expect MicF-Regulated-LuxI to produce more OHHL at low osmolarity conditions than at high osmolarity.
+Due to time constraints iGEM Stockholm 2015 were unable to construct this part.
 <!-- Add more about the biology of this part here