Difference between revisions of "Part:BBa K1587003"

Latest revision as of 20:40, 18 September 2015

3-hydroxybutyryl-CoA dehydratase

Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of Clostridium acetobutylicum. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1).

Figure 1: Crotonyl-CoA formation catalyzed by Crt

We used BBa_K1587003 with other genes to produce butyrate in Escherichia coli (Figure 2).

Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 609
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1587003 short</partinfo>
-Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of ''Clostridium acetobutylicum''. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA.
+Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of ''Clostridium acetobutylicum''. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1).
 <center>[[File:TLSE crt.jpg]]</center>
-<center>'''Figure 1 Crotonyl-CoA formation catalyzed by Crt'''</center>
+<center>'''Figure 1: Crotonyl-CoA formation catalyzed by Crt'''</center>
+We used [https://parts.igem.org/Part:BBa_K1587003 BBa_K1587003] with other genes to produce butyrate in ''Escherichia coli'' (Figure 2).
+<center>[[File:TLSE ButMetabo.jpg]]</center>
+<center>'''Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in ''E. coli'''''</center>
 <!-- Add more about the biology of this part here