Difference between revisions of "Part:BBa K1723003:Experience"

 
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
 
−
how you used this part and how it worked out.
+
  
 
===Applications of BBa_K1723003===
 
===Applications of BBa_K1723003===
 +
 +
<br/><br/><br/>
 +
https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png
 +
 +
<h3>This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h3>
 +
 +
<br/><br/><br/>
 +
 +
<h3> Effects of BBa_K1723000 on BBa_K1723001 promoter </h3>
 +
https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png
 +
<p>promoter strength was assessed using GFPmut2 reporter.</p>
 +
<br/>
 +
<p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/><br/>
 +
 +
[A] sgRNA Z4 (BBa_K1723003), activating<br/>
 +
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting<br/>
 +
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting<br/>
 +
[R2] sgRNA Z0 (BBa_K1723002), inhibiting<br/>
 +
[R1] sgRNA Z35 (BBa_K1723004), inhibiting<br/> <br/>
 +
 +
In comparison with:<br/> <br/>
 +
 +
[B] apo-dCas9-ω (=no sgRNA) <br/>
 +
[Af] apo-dCas9-ω (=no sgRNA), no reporter<br/>
 +
 +
</p>
 +
 +
<br/>
 +
<h3>This chart show the promoter PAM rich URS J23117 (BBa_K1723001) strength in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723003-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne</h3>
 +
<br/><br/>
 +
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 20:32, 18 September 2015


Applications of BBa_K1723003




EPF_Lausanne_transistor.png

This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.




Effects of BBa_K1723000 on BBa_K1723001 promoter

EPF_Lausanne_RESULTS_EXPERIENCE.png

promoter strength was assessed using GFPmut2 reporter.


This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:

[A] sgRNA Z4 (BBa_K1723003), activating
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
[R2] sgRNA Z0 (BBa_K1723002), inhibiting
[R1] sgRNA Z35 (BBa_K1723004), inhibiting

In comparison with:

[B] apo-dCas9-ω (=no sgRNA)
[Af] apo-dCas9-ω (=no sgRNA), no reporter


This chart show the promoter PAM rich URS J23117 (BBa_K1723001) strength in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723003-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne




User Reviews

UNIQ1652ea17307dd1c1-partinfo-00000000-QINU UNIQ1652ea17307dd1c1-partinfo-00000001-QINU