Difference between revisions of "Part:BBa K1595024"
 
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<partinfo>BBa_K1595024 parameters</partinfo>
 
<partinfo>BBa_K1595024 parameters</partinfo>
 
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<h3>Overview of Characterization of MBP+EcnB BioBrick</h3>
 
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As described on the <a href="http://2012.igem.org/Team:Cornell/testing/project/wetlab/2">2012 Cornell iGEM wiki</a> section, <i>S. oneidensis</i> MR-1 is capable of shuttling electrons through the Mtr pathway to reduce extracellular metals because of the negative free energy change associated with these redox reactions. Thus, to encourage <i>Shewanella</i> to transfer electrons to an electrode, we poise the potential of an electrode in a three electrode system, controlled by a potentiostat, so that electron transfer is energetically favorable to the organism. In short, a potentiostat works by setting the potential of a working electrode (WE) with respect to a Ag/AgCl reference electrode (RE) by injecting current through a counter electrode (CE).
 

Latest revision as of 20:25, 18 September 2015

MBP with EcnB from Mannheimia haemolytica

T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Mannheimia haemolytica EcnB with a stabilization maltose-binding protein and TEV cutsite.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138