Difference between revisions of "Part:BBa K1595024"
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<partinfo>BBa_K1595024 short</partinfo>
 
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T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Mannheimia haemolytica EcnB with a stabilizing maltose-binding protein and TEV cutsite.
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T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Mannheimia haemolytica EcnB with a stabilization maltose-binding protein and TEV cutsite.  
  
 
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<h3>Overview of Characterization of MBP+EcnB BioBrick</h3>
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As described on the <a href="http://2012.igem.org/Team:Cornell/testing/project/wetlab/2">2012 Cornell iGEM wiki</a> section, <i>S. oneidensis</i> MR-1 is capable of shuttling electrons through the Mtr pathway to reduce extracellular metals because of the negative free energy change associated with these redox reactions. Thus, to encourage <i>Shewanella</i> to transfer electrons to an electrode, we poise the potential of an electrode in a three electrode system, controlled by a potentiostat, so that electron transfer is energetically favorable to the organism. In short, a potentiostat works by setting the potential of a working electrode (WE) with respect to a Ag/AgCl reference electrode (RE) by injecting current through a counter electrode (CE).

Revision as of 20:23, 18 September 2015

MBP with EcnB from Mannheimia haemolytica

T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Mannheimia haemolytica EcnB with a stabilization maltose-binding protein and TEV cutsite.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138


Overview of Characterization of MBP+EcnB BioBrick

As described on the <a href="http://2012.igem.org/Team:Cornell/testing/project/wetlab/2">2012 Cornell iGEM wiki</a> section, S. oneidensis MR-1 is capable of shuttling electrons through the Mtr pathway to reduce extracellular metals because of the negative free energy change associated with these redox reactions. Thus, to encourage Shewanella to transfer electrons to an electrode, we poise the potential of an electrode in a three electrode system, controlled by a potentiostat, so that electron transfer is energetically favorable to the organism. In short, a potentiostat works by setting the potential of a working electrode (WE) with respect to a Ag/AgCl reference electrode (RE) by injecting current through a counter electrode (CE).