Difference between revisions of "Part:BBa K1795020"
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<partinfo>BBa_K1795020 short</partinfo> | <partinfo>BBa_K1795020 short</partinfo> | ||
| − | Cyan fluorescent protein with LVA fast degradation tag under the control of strong lambda cl regulated promoter R0051. | + | Cyan fluorescent protein with LVA fast degradation tag under the control of strong lambda cl regulated promoter R0051. This part has been visualized both via plasmid-based expression and genome integration expression. Confocal microscopy (60x oil immersion objective on a Nikon A1R confocal) shows strong fluorescent signal even for single copy number expression. See merged CFP/YFP image: [https://static.igem.org/mediawiki/parts/2/2d/150912_r0051_replicate_1_picture_6.png] |
Latest revision as of 20:21, 18 September 2015
CFP-LVA under promoter R0051
Cyan fluorescent protein with LVA fast degradation tag under the control of strong lambda cl regulated promoter R0051. This part has been visualized both via plasmid-based expression and genome integration expression. Confocal microscopy (60x oil immersion objective on a Nikon A1R confocal) shows strong fluorescent signal even for single copy number expression. See merged CFP/YFP image: [1]
![[1]](https://static.igem.org/mediawiki/parts/2/2d/150912_r0051_replicate_1_picture_6.png){kind=link}
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]