Difference between revisions of "Part:BBa J52008:Design"

 
(Source)
 
(2 intermediate revisions by one other user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
Rluc is cloned in BioBrick vector pSB1AK3.
+
Rluc is cloned in BioBrick vector pSB1AK3. We had no problems with making this part.
 
+
 
+
  
 
===Source===
 
===Source===
  
-
+
Our source for ''Renilla'' luciferase was vector phRL-TK from Promega.
  
 
===References===
 
===References===

Latest revision as of 15:28, 28 March 2007


luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 547


Design Notes

Rluc is cloned in BioBrick vector pSB1AK3. We had no problems with making this part.

Source

Our source for Renilla luciferase was vector phRL-TK from Promega.

References