Difference between revisions of "Part:BBa K1795015"
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<partinfo>BBa_K1795015 short</partinfo> | <partinfo>BBa_K1795015 short</partinfo> | ||
| − | Cyan fluorescent protein with LVA fast degradation tag under the control of strong lacI regulated promoter R0011. | + | Cyan fluorescent protein with LVA fast degradation tag under the control of strong lacI regulated promoter R0011. This part has been visualized both via plasmid-based expression and genome integration expression. Confocal microscopy (60x oil immersion objective on a Nikon A1R confocal) shows strong fluorescent signal even for single copy number expression. See merged CFP/YFP image: [https://static.igem.org/mediawiki/2015/8/89/WM150914_r0011_no_saturation_replicate_1_picture_4.png] |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 20:16, 18 September 2015
CFP-LVA under promoter R0011
Cyan fluorescent protein with LVA fast degradation tag under the control of strong lacI regulated promoter R0011. This part has been visualized both via plasmid-based expression and genome integration expression. Confocal microscopy (60x oil immersion objective on a Nikon A1R confocal) shows strong fluorescent signal even for single copy number expression. See merged CFP/YFP image: [1] Sequence and Features
![[1]](https://static.igem.org/mediawiki/2015/8/89/WM150914_r0011_no_saturation_replicate_1_picture_4.png){kind=link}
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]