Difference between revisions of "Part:BBa K1679009:Design"

m (→‎References)
(→‎Design Notes)
 
(3 intermediate revisions by one other user not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
We use a special RBS to make the device temperature-sensitive.When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded.Thus the ribosome can't translated RFP.And when the temperature rises above 37°C,the secondary structure of the RBS which after transform would be unfolded and produce RFP.However,according to our experimental,it didn't appear red when the temperature rises above 37°C.
+
We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 37°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.
  
 
===Source===
 
===Source===
Line 14: Line 14:
  
 
BBa_E1010
 
BBa_E1010
−
 
−
BBa_B0015
 
  
 
===References===
 
===References===
 
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.
 
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.

Latest revision as of 19:59, 18 September 2015

promoter+RNA thermometer+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 713
    Illegal AgeI site found at 825
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 37°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.

Source

BBa_J23101

BBa_K115003

BBa_E1010

References

Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.