Difference between revisions of "Part:BBa K1763425:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
This construct was assembled by subcloning [https://parts.igem.org/Part:BBa_K1763424 BBa_K1763424] into [https://parts.igem.org/Part:BBa_K525998].  
+
We chose to express [https://parts.igem.org/Part:BBa_K1763424 BBa_K1763424] under control of the T7-RBS system rather than the Lac operon due to our access to well characterized BL21(DE3) expression systems.
 +
 
 +
This construct was assembled by subcloning [https://parts.igem.org/Part:BBa_K1763424 BBa_K1763424] into [https://parts.igem.org/Part:BBa_K525998 BBa_K525998].  
  
 
This part was sequence verified using registry standard VF2 and VR primers.
 
This part was sequence verified using registry standard VF2 and VR primers.
  
 
===Source===
 
===Source===
−
 
+
[https://parts.igem.org/Part:BBa_K1763424 BBa_K1763424] was created by our team.
−
Nephila clavipes
+
[https://parts.igem.org/Part:BBa_K525998 BBa_K525998] was obtained through the 2015 Registry Distribution.
  
 
===References===
 
===References===
 +
Briggs A., Rios X., Chari R., Luhan Y., Zhang F., Mali P., and Church G. Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. ''Nucleic Acids Research''. 2012;'''40'''(15): e117
 +
 +
Hinman, M.B., Lewis, R. Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber. ''J. Biol. Chem''. 1992;'''267''': 19320–19324.
 +
 +
Tokareva O., Michalczechen-Lacerda V., Rech E., and Kaplan D. Recombinant DNA production of spider silk proteins. ''Microbial Biotechnology''. 2013;'''6'''(6): 651-663
 +
 +
Xu, M., Lewis, R.V. Structure of a protein superfiber: spider dragline silk. ''PNAS'';1990:'''87''': 7120.

Latest revision as of 19:56, 18 September 2015


MaSp2-3(T7)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose to express BBa_K1763424 under control of the T7-RBS system rather than the Lac operon due to our access to well characterized BL21(DE3) expression systems.

This construct was assembled by subcloning BBa_K1763424 into BBa_K525998.

This part was sequence verified using registry standard VF2 and VR primers.

Source

BBa_K1763424 was created by our team. BBa_K525998 was obtained through the 2015 Registry Distribution.

References

Briggs A., Rios X., Chari R., Luhan Y., Zhang F., Mali P., and Church G. Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. Nucleic Acids Research. 2012;40(15): e117

Hinman, M.B., Lewis, R. Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber. J. Biol. Chem. 1992;267: 19320–19324.

Tokareva O., Michalczechen-Lacerda V., Rech E., and Kaplan D. Recombinant DNA production of spider silk proteins. Microbial Biotechnology. 2013;6(6): 651-663

Xu, M., Lewis, R.V. Structure of a protein superfiber: spider dragline silk. PNAS;1990:87: 7120.