Difference between revisions of "Part:BBa K1595020"
 
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<partinfo>BBa_K1595020 short</partinfo>
 
<partinfo>BBa_K1595020 short</partinfo>
  
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Coding device for anti-microbial peptide derived from Ralstonia pickettii EcnB with a stabilizing maltose-binding protein and TEV cutsite.
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T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Ralstonia pickettii EcnB with a stabilizing maltose-binding protein and TEV cutsite.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:34, 18 September 2015

MBP with EcnB from Ralstonia pickettii.

T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Ralstonia pickettii EcnB with a stabilizing maltose-binding protein and TEV cutsite.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1257
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138