Difference between revisions of "Part:BBa K1820000"
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− | <partinfo> | + | <partinfo>BBa_K1820014 short</partinfo> |
+ | <p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites.</p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <p> Transcption form this promoter is induced after phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.</p> | ||
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− | <span class='h3bb'> | + | |
− | <partinfo> | + | ===Sequence and Features=== |
+ | <span class='h3bb'></span> | ||
+ | <partinfo>BBa_K1820014 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K1820014 parameters</partinfo> |
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+ | ===References=== | ||
+ | <p>Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.</p> | ||
+ | <p>Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.</p> |
Revision as of 19:26, 18 September 2015
CP8_RBS_sfGFP(Bs)_Terminator
A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites.
Usage and Biology
Transcption form this promoter is induced after phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 99
References
Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.
Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.