Difference between revisions of "Part:BBa K1602009"
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===<h2>Sequence and Features</h2>===
 
===<h2>Sequence and Features</h2>===
 
<partinfo>BBa_K1602009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1602009 SequenceAndFeatures</partinfo>
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<br>
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===References===
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1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.

Revision as of 19:16, 18 September 2015

D-Xylose dehydrogenase - xylB

xylB is coding for a NAD+ D-xylose dehydrogenase from Caulobacter crescentus. It catalyzes the reaction of D-xylose to D-xylonolactone. The sequence is codon optimized for the usage in E. coli.
https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png

Characteristics


Molecular Weight 26641.48
Residues 248
Charge -4.0
Isoelectric Point 5.1521
A280 Molar Extinction Coefficients 36440 (reduced) 36690 (cystine bridges)
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.