Revision as of 19:14, 18 September 2015

Lpp-OmpA

Introduction

Lpp-OmpA protein is a the expression system of outer membrane protein, which consists of the 20 amino acids of signal sequence, the nine N-terminal amino acids of the lipoprotein (Lpp) and the residual 46-159 amino acids of the OmpA.
The lipoprotein (Lpp) is the most abundant protein on the outer membrane, which possesses the function of targeting to the outer membrane.
The OmpA domain constitutes eight-stranded β-barrel to construct an anchor on the outer membrane, providing the stable expression of the scFv.
By taking advantage of efficient targeting OmpA to the outer membrane, it allows C-terminal fusion of the scFv sequence to be displayed out of the outer mambrane.

Experiment

After PCR the DNA sequences from Lpp-OmpA-N , we get the Lpp-OmpA, and then recombined each Lpp-OmpA gene to PSB1C3 backbones and conducted a PCR experiment to check the size of each of the Lpp-OmpA. The DNA sequence length of the Lpp-OmpA is around 400~500 bp. In this PCR experiment, the Lpp-OmpA products size should be near at 650~750 bp. The Fig. showed the correct size of the Lpp-OmpA, and proved that we successful ligated the Lpp-OmpA sequence onto an ideal backbone.

Sequence and Features