Revision as of 19:10, 18 September 2015

Status: 500 Content-type: text/html

Software error:

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Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
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The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The RhlA gene (Ba_K1688002) codes for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the RhlB (BBa_K1688003) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick BBa_K1688000 is a composite part of a promotor BBa_J23101 and BBa_K1688001 which transcribes the genes RhlA and RhlB into mRNA which eventually leads to translation of proteins that induce mono-rhamnolipid production.

Sequence and Features Status: 500 Content-type: text/html

Software error:

Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

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Gel electrophoresis Table 1: Biobricks used for gel electrophoresis, their inserts, restrictions enzymes used for digestion, lengths of inserts and plasmid backbones and expected band lengths.

Biobrick Code Insert Digestion Insert (bp)Backbone (bp)Expected bands BBa_K1688000 Promoter + RBS + Rhl A + RBS + Rhl B EcoRI, PstI 2333 2070 2374, 2037 BBa_K1688001 RBS + Rhl A + RBS + Rhl B XbaI, PstI 2333 2070 2324, 2052 BBa_K1688002 RBS + Rhl A EcoRI, PstI 2298 2070 1006, 2037 BBa_K1688003 RBS + Rhl B EcoRI, PstI 1325 2070 1366, 2037