Difference between revisions of "Part:BBa K567018:Experience"

Revision as of 19:05, 18 September 2015

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Applications of BBa_K567018

User Reviews

UNIQba1d3891dc392edc-partinfo-00000000-QINU

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kosyumote

The Pitt iGEM 2015 team (Group: iGEM15_Pitt (2015-09-18)) characterized this part in NiCo21(DE3) cells (a BL21(DE3) derivative). This part exhibits significant leaky expression due to the lac operator present in high copy number. For applications where leaky expression is deleterious, this part does not work. However, it produces easily quantifiable GFP which can be useful in many applications. Finally, we did not expect RFP fluorescence in NiCo21(DE3) or DH5a cells, since neither of them have amber suppressors, and none was observed.

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UNIQba1d3891dc392edc-partinfo-00000002-QINU

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 <I>kosyumote</I>
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-The Pitt iGEM 2015 team characterized this part in NiCo21(DE3) cells (a BL21(DE3) derivative). This part exhibits significant leaky expression due to the lac operator present in high copy number. For applications where leaky expression is deleterious, this part does not work. However, it produces easily quantifiable GFP which can be useful in many applications. Finally, we did not expect RFP fluorescence in NiCo21(DE3) or DH5a cells, since neither of them have amber suppressors, and none was observed.
+The Pitt iGEM 2015 team (Group: iGEM15_Pitt (2015-09-18)) characterized this part in NiCo21(DE3) cells (a BL21(DE3) derivative). This part exhibits significant leaky expression due to the lac operator present in high copy number. For applications where leaky expression is deleterious, this part does not work. However, it produces easily quantifiable GFP which can be useful in many applications. Finally, we did not expect RFP fluorescence in NiCo21(DE3) or DH5a cells, since neither of them have amber suppressors, and none was observed.
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