Difference between revisions of "Part:BBa K1688018"

 
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<partinfo>BBa_K1688018 short</partinfo>
 
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Upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate.
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Upper naphthalene breakdown pathway from the Nah7 plasmid of ''Pseudomonas putida''. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate. The mutagenized pathway no longer contains the two unwanted PstI restriction sites in the coding sequence.
  
 
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Revision as of 18:56, 18 September 2015

Nah7 - Upper naphthalene breakdown pathway mutagenized

Upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate. The mutagenized pathway no longer contains the two unwanted PstI restriction sites in the coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4119
    Illegal NotI site found at 8162
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5212
    Illegal BamHI site found at 3197
    Illegal BamHI site found at 5899
    Illegal XhoI site found at 1308
    Illegal XhoI site found at 3696
    Illegal XhoI site found at 3848
    Illegal XhoI site found at 9350
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1801
    Illegal NgoMIV site found at 3576
    Illegal NgoMIV site found at 5450
    Illegal NgoMIV site found at 6062
    Illegal NgoMIV site found at 9375
    Illegal AgeI site found at 639
    Illegal AgeI site found at 3570
    Illegal AgeI site found at 6278
    Illegal AgeI site found at 8273
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2918
    Illegal BsaI site found at 3591
    Illegal BsaI site found at 4182
    Illegal BsaI site found at 5348
    Illegal BsaI.rc site found at 2752
    Illegal BsaI.rc site found at 5502
    Illegal BsaI.rc site found at 7787
    Illegal SapI.rc site found at 3282
    Illegal SapI.rc site found at 3828
    Illegal SapI.rc site found at 7679