Difference between revisions of "Part:BBa K1819001"
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<p>Figure 1 - Schematic representation of BBa_K1819001 insert</p> | <p>Figure 1 - Schematic representation of BBa_K1819001 insert</p> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/b/b8/Team-Brasil-USP-LCP-PSB1C3.png" width= " | + | <img src="https://static.igem.org/mediawiki/2015/b/b8/Team-Brasil-USP-LCP-PSB1C3.png" width= "300"> </img src> |
<p>figure 2 - Gel analysis confirmation of Lcp in pSB1C3</p> | <p>figure 2 - Gel analysis confirmation of Lcp in pSB1C3</p> | ||
Revision as of 18:50, 18 September 2015
Latex Clearing Protein (Lcp) - rubber degradation
Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg.
It contains an oxidized heme-Fe3+ not bound to dioxygen.
Figure 1 - Schematic representation of BBa_K1819001 insert
figure 2 - Gel analysis confirmation of Lcp in pSB1C3
Please note this part was submitted in pSB1C3, the Registry's standard shipping backbone, according to submission requirements. However, pSB1C3 contains a SacI site and if you want to use this part we recommend to move the part into another plasmid backbone what can be easily done.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 889
Illegal XhoI site found at 1043 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 535
Illegal NgoMIV site found at 857 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 438