Difference between revisions of "Part:BBa K1714001"
 
(8 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1714001 short</partinfo>
 
<partinfo>BBa_K1714001 short</partinfo>
  
−
This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.
+
https://static.igem.org/mediawiki/2015/6/66/Hokkaidou_co-expression_Fig.5.png
−
In this page, I described the result of the activity assay of ABF-2.
+
  
  
 +
This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.
 
In this page, I described the result of the activity assay of ABF-2.
 
In this page, I described the result of the activity assay of ABF-2.
  
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K1714001 SequenceAndFeatures</partinfo>
  
  
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K1714001 parameters</partinfo>
 +
<!-- -->
  
 +
 +
In this page, I described the result of the activity assay of ABF-2.
  
 
We transformed it into <i>E. coli</i> (in this case, we used DH5α) and compared the growth curve between samples induced or not.
 
We transformed it into <i>E. coli</i> (in this case, we used DH5α) and compared the growth curve between samples induced or not.
Line 22: Line 31:
  
 
As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.
 
As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.
 +
 +
https://static.igem.org/mediawiki/2015/b/ba/Hokkaido_U_ABF-2_chart1.png
  
  

Latest revision as of 18:37, 18 September 2015

pLac-RBS B0034-ABF-2-double terminator

Hokkaidou_co-expression_Fig.5.png


This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system. In this page, I described the result of the activity assay of ABF-2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 321
  • 1000
    COMPATIBLE WITH RFC[1000]



In this page, I described the result of the activity assay of ABF-2.

We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not. Activity test for ABF-2 and thanatin →Comparing growth curve between the samples one is induced by IPTG and the other is not induced.

  1. Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
  2. Adding 2 µL of IPTG into one and DW into the other.
  3. Measuring OD600 of these samples once in an hour.

As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.

Hokkaido_U_ABF-2_chart1.png


Fig. 8. The growth curve of E. coli including BBa_K1714001 with IPTG or not.

As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity.