Difference between revisions of "Part:BBa K1714001"

(Created page with "<p>This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system. In this page, I described the result of...")
 
 
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<p>This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.
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__NOTOC__
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In this page, I described the result of the activity assay of ABF-2.</p>
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<partinfo>BBa_K1714001 short</partinfo>
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https://static.igem.org/mediawiki/2015/6/66/Hokkaidou_co-expression_Fig.5.png
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This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.
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In this page, I described the result of the activity assay of ABF-2.
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1714001 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K1714001 parameters</partinfo>
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<!-- -->
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In this page, I described the result of the activity assay of ABF-2.
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We transformed it into <i>E. coli</i> (in this case, we used DH5α) and compared the growth curve between samples induced or not.
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Activity test for ABF-2 and thanatin
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→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.
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  <ol>
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  <li>Diluting the bacterial culture which is enough pre-cultured by 0.1 OD<sub>600</sub> (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol) </li>
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  <li>Adding 2 µL of IPTG into one and DW into the other.</li>
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  <li>Measuring OD<sub>600</sub> of these samples once in an hour. </li>
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  </ol>
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As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.
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https://static.igem.org/mediawiki/2015/b/ba/Hokkaido_U_ABF-2_chart1.png
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Fig. 8. The growth curve of <i>E. coli</i> including BBa_K1714001 with IPTG or not.
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As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity.

Latest revision as of 18:37, 18 September 2015

pLac-RBS B0034-ABF-2-double terminator

Hokkaidou_co-expression_Fig.5.png


This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system. In this page, I described the result of the activity assay of ABF-2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 321
  • 1000
    COMPATIBLE WITH RFC[1000]



In this page, I described the result of the activity assay of ABF-2.

We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not. Activity test for ABF-2 and thanatin →Comparing growth curve between the samples one is induced by IPTG and the other is not induced.

  1. Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
  2. Adding 2 µL of IPTG into one and DW into the other.
  3. Measuring OD600 of these samples once in an hour.

As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.

Hokkaido_U_ABF-2_chart1.png


Fig. 8. The growth curve of E. coli including BBa_K1714001 with IPTG or not.

As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity.