Difference between revisions of "Part:BBa K1723007:Design"
E.Cuillery (Talk | contribs) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1723007 short</partinfo> | <partinfo>BBa_K1723007 short</partinfo> | ||
| Line 8: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | This part was design on the model for sgRNAs on the paper from Alec AK Nielsen & Christopher A Voigt [2] and the specific sequence for the gRNA X4 was designed from the sequence of the PAM rich URS J23117Alt promoter (BBa_K1723005). | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| Line 17: | Line 14: | ||
===References=== | ===References=== | ||
| + | |||
| + | [1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437. | ||
| + | |||
| + | [2] Alec AK Nielsen & Christopher A Voigt (2014). Multi-input CRISPR/Cas circuits that interface host regulatory network. Molecular systems biology, 10(11), 763. | ||
Latest revision as of 17:56, 18 September 2015
sgRNA X4 expressing cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 126
Illegal XhoI site found at 185 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was design on the model for sgRNAs on the paper from Alec AK Nielsen & Christopher A Voigt [2] and the specific sequence for the gRNA X4 was designed from the sequence of the PAM rich URS J23117Alt promoter (BBa_K1723005).
Source
this part was fully synthesized.
References
[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.
[2] Alec AK Nielsen & Christopher A Voigt (2014). Multi-input CRISPR/Cas circuits that interface host regulatory network. Molecular systems biology, 10(11), 763.