Difference between revisions of "Part:BBa K1709001:Design"
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===Design Notes=== | ===Design Notes=== | ||
Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. Also, a Ribosome Binding Site was added. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding. | Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. Also, a Ribosome Binding Site was added. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding. | ||
| − | + | gBlocks were used to partly design the plasmid and the ribosome binding site sequence was based purely on the Anderson library and thus, there is a mistake in the composite part sequence since the scar is only partly present. | |
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 17:26, 18 September 2015
LuxI-His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. Also, a Ribosome Binding Site was added. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding. gBlocks were used to partly design the plasmid and the ribosome binding site sequence was based purely on the Anderson library and thus, there is a mistake in the composite part sequence since the scar is only partly present.
Source
Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.