Difference between revisions of "Part:BBa K1714001"
| Line 15: | Line 15: | ||
As a result, we got the graph of growth curve of DH5α and shown on below as Chart1. | As a result, we got the graph of growth curve of DH5α and shown on below as Chart1. | ||
| − | "https://static.igem.org/mediawiki/2015/b/ba/Hokkaido_U_ABF-2_chart1.png" width= | + | "https://static.igem.org/mediawiki/2015/b/ba/Hokkaido_U_ABF-2_chart1.png" width=60% class="figure" |
Fig. 8. The growth curve of <i>E. coli</i> including BBa_K1714001 with IPTG or not. | Fig. 8. The growth curve of <i>E. coli</i> including BBa_K1714001 with IPTG or not. | ||
As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity. | As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity. | ||
Revision as of 17:21, 18 September 2015
This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system. In this page, I described the result of the activity assay of ABF-2.
In this page, I described the result of the activity assay of ABF-2.
We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not. Activity test for ABF-2 and thanatin →Comparing growth curve between the samples one is induced by IPTG and the other is not induced.
- Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
- Adding 2 µL of IPTG into one and DW into the other.
- Measuring OD600 of these samples once in an hour.
As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.
"
" width=60% class="figure"
Fig. 8. The growth curve of E. coli including BBa_K1714001 with IPTG or not.
As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity.