Difference between revisions of "Part:BBa K1714001"
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<p>As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.</p>
 
<p>As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.</p>
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<img src="https://static.igem.org/mediawiki/2015/b/ba/Hokkaido_U_ABF-2_chart1.png" class="figure">
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<p class="caption">Fig. 8. The growth curve of <i>E. coli</i> including <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1714001" target="blank">BBa_K1714001</a> with IPTG or not </p>
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<p>As you can see, we can’t say that ABF-2 in
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  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1714001" target="blank">BBa_K1714001</a>
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  has correct antimicrobial activity.</p>

Revision as of 17:16, 18 September 2015

This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system. In this page, I described the result of the activity assay of ABF-2.

In this page, I described the result of the activity assay of ABF-2.

We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not.

Activity test for ABF-2 and thanatin

→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.

  1. Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
  2. Adding 2 µL of IPTG into one and DW into the other.
  3. Measuring OD600 of these samples once in an hour.

As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.

<img src="Hokkaido_U_ABF-2_chart1.png" class="figure">

Fig. 8. The growth curve of E. coli including <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1714001" target="blank">BBa_K1714001</a> with IPTG or not

As you can see, we can’t say that ABF-2 in <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1714001" target="blank">BBa_K1714001</a> has correct antimicrobial activity.