Difference between revisions of "Part:BBa K1590003"

Revision as of 17:15, 18 September 2015

Chromate responsive promoter

The promoter pChr is suspected to be inducible by chromate.

Sequence and Features

Assembly Compatibility:

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 ===Usage and Biology===
+This promoter is found upstream of the <i>ChrBACF</i> - operon in <i>Ochrobactrum tritici</i> 5bvl1, located in the transposable element Tn<i>OtChr</i> of 7189bp length.
+Ochrobactrum tritici 5bvl1 was discovered in 2004 in a chromate contaminated wastewater treatment facility. A specific characteristic of this strain was that it could both, reduce Cr(VI) and be resistant to it at the same time. Previously characterised strains were either resistant or could reduce chromate. Over the last 10 years the group that discovered it started working out the underlying mechanisms of this set of characteristics.
+The <i>ChrBACF</i> - operon, expressed from the P<i>chr</i> - promoter led to the expression of the downstream genes <i>chrA, chrB, chrC</i>, and <i>chrF</i>. <i>chrA</i> was found to express and efflux pump, whereas the gene product of <i>chrB</i> was identified as being a chromate-inducible regulator of P<i>chr</i>. Additionally, <i>chrC</i> and <i>chrF</i> were found to confer tolerance to superoxides.
+P<i>chr itself is believed to be regulated by the chromate-sensitive repressor <i>chrB</i>. This has made it a candidate for a biological sensor of chromate contamination in e.g. rivers (Branco et al.), or in milk (iGEM BIT 2013).
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