Difference between revisions of "Part:BBa K1723000:Experience"
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===Applications of BBa_K1723000=== | ===Applications of BBa_K1723000=== | ||
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https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png | https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png | ||
<h4>This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h4> | <h4>This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h4> | ||
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<h3> Effects of BBa_K1723000 on BBa_K1723001 promoter </h3> | <h3> Effects of BBa_K1723000 on BBa_K1723001 promoter </h3> | ||
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<h3> Effects of BBa_K1723000 on BBa_K1723005 promoter </h3> | <h3> Effects of BBa_K1723000 on BBa_K1723005 promoter </h3> | ||
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</p> | </p> | ||
| + | <h2>Those charts show that BBa_K1723000 can act as a transcription regulator on both promoters BBa_K1723001 and BBa_K1723005 by either activating on inhibiting them.</h2> | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:53, 18 September 2015
Applications of BBa_K1723000
This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.
Effects of BBa_K1723000 on BBa_K1723001 promoter
This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:
[A] sgRNA Z4 (BBa_K1723003), activating
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
[R2] sgRNA Z0 (BBa_K1723002), inhibiting
[R1] sgRNA Z35 (BBa_K1723004), inhibiting
In comparison with:
[B] apo-dCas9-ω (=no sgRNA)
[Af] no dCas9-ω, no sgRNA
Effects of BBa_K1723000 on BBa_K1723005 promoter
This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:
[A_alt] sgRNA X4 (BBa_K1723007), activating
[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting
[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting
In comparison with:
[B_alt] apo-dCas9-ω (=no sgRNA)
[Af_alt] no dCas9-ω, no sgRNA
Those charts show that BBa_K1723000 can act as a transcription regulator on both promoters BBa_K1723001 and BBa_K1723005 by either activating on inhibiting them.
User Reviews
UNIQ6a2a195d7891ad58-partinfo-00000000-QINU UNIQ6a2a195d7891ad58-partinfo-00000001-QINU