Difference between revisions of "Part:BBa K1723000:Experience"
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===Applications of BBa_K1723000===
 
===Applications of BBa_K1723000===
 +
<br/><br/>
  
 
https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png
 
https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png
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<h4>This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h4>
 
<h4>This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h4>
  
 +
<h3> Effects of BBa_K1723000 on BBa_K1723001 promoter </h3>
 
https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png
 
https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png
 
<p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/><br/>
 
<p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/><br/>
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In comparison with:<br/> <br/>
 
In comparison with:<br/> <br/>
  
−
[B] The basal level, apo-dCas9-ω (no sgRNA) <br/>
+
[B] apo-dCas9-ω (=no sgRNA) <br/>
−
[Af]The auto fluorescence level, no dCas9-ω<br/>
+
[Af] no dCas9-ω, no sgRNA<br/>
  
 +
</p>
  
 +
<h3> Effects of BBa_K1723000 on BBa_K1723005 promoter </h3>
 +
https://static.igem.org/mediawiki/2015/0/09/EPF_Lausanne_RESULTS_EXPERIENCE2.png
  
 +
<p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:<br/><br/>
  
−
</p>
+
[A_alt] sgRNA X4 (BBa_K1723007), activating<br/>
 +
[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting<br/>
 +
[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting<br/> <br/>
  
−
https://static.igem.org/mediawiki/2015/0/09/EPF_Lausanne_RESULTS_EXPERIENCE2.png
+
In comparison with:<br/> <br/>
  
 +
[B_alt] apo-dCas9-ω (=no sgRNA) <br/>
 +
[Af_alt] no dCas9-ω, no sgRNA<br/>
 +
 +
</p>
  
  

Revision as of 16:50, 18 September 2015


Applications of BBa_K1723000



EPF_Lausanne_transistor.png

This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.

Effects of BBa_K1723000 on BBa_K1723001 promoter

EPF_Lausanne_RESULTS_EXPERIENCE.png

This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:

[A] sgRNA Z4 (BBa_K1723003), activating
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
[R2] sgRNA Z0 (BBa_K1723002), inhibiting
[R1] sgRNA Z35 (BBa_K1723004), inhibiting

In comparison with:

[B] apo-dCas9-ω (=no sgRNA)
[Af] no dCas9-ω, no sgRNA

Effects of BBa_K1723000 on BBa_K1723005 promoter

EPF_Lausanne_RESULTS_EXPERIENCE2.png

This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:

[A_alt] sgRNA X4 (BBa_K1723007), activating
[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting
[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting

In comparison with:

[B_alt] apo-dCas9-ω (=no sgRNA)
[Af_alt] no dCas9-ω, no sgRNA


User Reviews

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