Difference between revisions of "Part:BBa K1723000:Experience"
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https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png | https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png | ||
| − | < | + | <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:</br> |
| − | [A] sgRNA Z4 (BBa_K1723003), activating | + | [A] sgRNA Z4 (BBa_K1723003), activating</br> |
| − | [A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting | + | [A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting</br> |
| − | [A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting | + | [A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting</br> |
| − | [R2] sgRNA Z0 (BBa_K1723002), inhibiting | + | [R2] sgRNA Z0 (BBa_K1723002), inhibiting</br> |
| − | [R1] sgRNA Z35 (BBa_K1723004), inhibiting | + | [R1] sgRNA Z35 (BBa_K1723004), inhibiting</br> |
| − | In comparison with: | + | In comparison with:</br> |
| − | [B] | + | [B] </br> |
| − | [Af] | + | [Af]</br> |
| − | </ | + | </p> |
https://static.igem.org/mediawiki/2015/0/09/EPF_Lausanne_RESULTS_EXPERIENCE2.png | https://static.igem.org/mediawiki/2015/0/09/EPF_Lausanne_RESULTS_EXPERIENCE2.png | ||
Revision as of 16:39, 18 September 2015
Applications of BBa_K1723000
This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.
This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:</br> [A] sgRNA Z4 (BBa_K1723003), activating</br> [A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting</br> [A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting</br> [R2] sgRNA Z0 (BBa_K1723002), inhibiting</br> [R1] sgRNA Z35 (BBa_K1723004), inhibiting</br> In comparison with:</br> [B] </br> [Af]</br>
User Reviews
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