Difference between revisions of "Part:BBa K1723000:Experience"
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https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png
 
https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png
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<p></p>
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<h2>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:
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[A] sgRNA Z4 (BBa_K1723003), activating
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[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
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[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
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[R2] sgRNA Z0 (BBa_K1723002), inhibiting
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[R1] sgRNA Z35 (BBa_K1723004), inhibiting
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In comparison with:
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[B]
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[Af]
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</h2>
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https://static.igem.org/mediawiki/2015/0/09/EPF_Lausanne_RESULTS_EXPERIENCE2.png
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Revision as of 16:37, 18 September 2015


Applications of BBa_K1723000

EPF_Lausanne_transistor.png

This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.

EPF_Lausanne_RESULTS_EXPERIENCE.png

This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs: [A] sgRNA Z4 (BBa_K1723003), activating [A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting [A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting [R2] sgRNA Z0 (BBa_K1723002), inhibiting [R1] sgRNA Z35 (BBa_K1723004), inhibiting In comparison with: [B] [Af]

EPF_Lausanne_RESULTS_EXPERIENCE2.png


User Reviews

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