Difference between revisions of "Part:BBa K1758311:Design"
(Created page with "_NOTOC__ <partinfo>BBa_K1465102 short</partinfo> <partinfo>BBa_K1465102 SequenceAndFeatures</partinfo> ===Design Notes=== ===Source <html> The original source of our chromat...") |
|||
| Line 9: | Line 9: | ||
===Source | ===Source | ||
| + | |||
<html> | <html> | ||
The original source of our chromate responsive promoter is <i>Ochrobactrum tritici</i> 5bvl1. We used a codon optimized version which we got synthesised by IDT. | The original source of our chromate responsive promoter is <i>Ochrobactrum tritici</i> 5bvl1. We used a codon optimized version which we got synthesised by IDT. | ||
Revision as of 16:37, 18 September 2015
_NOTOC__ Fumarate reductase (frd) from E. coli under the control of the T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 666
Illegal AgeI site found at 741
Illegal AgeI site found at 3023 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
===Source
The original source of our chromate responsive promoter is Ochrobactrum tritici 5bvl1. We used a codon optimized version which we got synthesised by IDT.