Difference between revisions of "Part:BBa K515000"
 
(8 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K515000 short</partinfo>
 
<partinfo>BBa_K515000 short</partinfo>
−
 
+
<html>
−
IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. There are several different pathways to produce indole-3-acetic acid. [1] We are using genes from the IAM pathway (IaaM and IaaH) of the plant pathogen, <i>Psuedomonas savastanoi</i> to produce indole-3-acetic acid in <i>E. coli</i> </p>
+
IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. This auxin producing pathway originates from the plant pathogen, <i> Pseudomonas savastanoi </i> </p>
−
 
+
<p>This is one of the parts of our composite auxin expressing construct, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515100" target="_blank">BBa_K515100</a>.</p>
−
<p> tryptophan --> indole acetamide --> indole-3-acetic acid </p>
+
</html>
−
 
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 19: Line 18:
 
<partinfo>BBa_K515000 parameters</partinfo>
 
<partinfo>BBa_K515000 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
<html>
 +
<p><b>This BioBrick has been sequence verified.</b></p>
 +
<p><b>For the full characterisation of the device, please refer to the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515100"><b>BBa_K515100</b></a> page.</b>
  
 
<h2>References</h2>
 
<h2>References</h2>
 
<p>[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. <i>Federation of European Microbiological Societies Microbiology Reviews </i>, 31, pp.425–448 </p>
 
<p>[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. <i>Federation of European Microbiological Societies Microbiology Reviews </i>, 31, pp.425–448 </p>
 +
</html>
 +
 +
 +
==Contribution: NUDT_CHINA 2015==
 +
Author: Xinyuan Qiu
 +
 +
Summary: We built a new part based on this part to extend its function.
 +
 +
We designed a new part by removing the RBS from this part to extend its usage.
 +
 +
====A new Bio-brick was designed based on this part====
 +
 +
Primers used to build the new part:
 +
 +
F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’
 +
 +
R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’
 +
 +
 +
For further information,
 +
 +
See BBa_K1789000
 +
 +
This part was sent to the registry.

Latest revision as of 16:35, 18 September 2015

IaaM - tryptophan-2-mono-oxygenase IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. This auxin producing pathway originates from the plant pathogen, Pseudomonas savastanoi

This is one of the parts of our composite auxin expressing construct, BBa_K515100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

For the full characterisation of the device, please refer to the BBa_K515100 page.

References

[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. Federation of European Microbiological Societies Microbiology Reviews , 31, pp.425–448


Contribution: NUDT_CHINA 2015

Author: Xinyuan Qiu

Summary: We built a new part based on this part to extend its function.

We designed a new part by removing the RBS from this part to extend its usage.

A new Bio-brick was designed based on this part

Primers used to build the new part:

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’

R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’


For further information,

See BBa_K1789000

This part was sent to the registry.