Difference between revisions of "Part:BBa K515000"
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<h2>References</h2>
 
<h2>References</h2>
 
<p>[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. <i>Federation of European Microbiological Societies Microbiology Reviews </i>, 31, pp.425–448 </p>
 
<p>[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. <i>Federation of European Microbiological Societies Microbiology Reviews </i>, 31, pp.425–448 </p>
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==Contribution: NUDT_CHINA 2015==
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Author: Xinyuan Qiu
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Summary: We built a new part based on this part to extend its function.
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We designed a new part by removing the RBS from this part to extend its usage.
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====A new Bio-brick was designed based on this part====
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Primers used to build the new part:
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F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’
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R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’
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For further information,
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See BBa_K1789000
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This part was sent to the registry.

Revision as of 16:34, 18 September 2015

IaaM - tryptophan-2-mono-oxygenase IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. This auxin producing pathway originates from the plant pathogen, Pseudomonas savastanoi

This is one of the parts of our composite auxin expressing construct, BBa_K515100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

For the full characterisation of the device, please refer to the BBa_K515100 page.

References

[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. Federation of European Microbiological Societies Microbiology Reviews , 31, pp.425–448

==Contribution: NUDT_CHINA 2015== Author: Xinyuan Qiu Summary: We built a new part based on this part to extend its function. We designed a new part by removing the RBS from this part to extend its usage. ====A new Bio-brick was designed based on this part==== Primers used to build the new part: F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’ R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’ For further information, See BBa_K1789000 This part was sent to the registry.