Difference between revisions of "Part:BBa K1725301"
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Parts K1725301-K1725332 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR. | Parts K1725301-K1725332 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR. | ||
| + | ==B0032-derived RBS library members== | ||
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| − | + | <sup></sup> Randomised sequence locations are shown in red. Estimated strength corresponds to the Translation Initiation Rate values predicted by RBS library calculator for <i>luxA</i> gene. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 16:28, 18 September 2015
Member of the RBS library - derived from BBa_B0032
Parts K1725301-K1725332 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR.
B0032-derived RBS library members
| Identifier | Sequence | Estimated strength (au) |
|---|---|---|
| Degenerate sequence | TCACACANRARRG | - |
| BBa_K1725301 | TCACACAGGAGGG | 15730.86 |
| BBa_K1725302 | TCACACAGGAGAG | 12493.41 |
| BBa_K1725303 | TCACACAGGAAGG | 8716.05 |
| BBa_K1725304 | TCACACAAGAGGG | 7247.43 |
| BBa_K1725305 | TCACACAAGAAGG | 5532.39 |
| BBa_K1725306 | TCACACACGAGGG | 3880.58 |
| BBa_K1725307 | TCACACATGAGGG | 3880.58 |
| BBa_K1725308 | TCACACAAAAAGG | 2574.21 |
| BBa_K1725309 | TCACACAAAAGGG | 2574.21 |
| BBa_K1725310 | TCACACACAAAGG | 2574.21 |
| BBa_K1725311 | TCACACACAAGGG | 2574.21 |
| BBa_K1725312 | TCACACAGAAAGG | 2574.21 |
| BBa_K1725313 | TCACACATAAAGG | 2574.21 |
| BBa_K1725314 | TCACACATAAGGG | 2574.21 |
| BBa_K1725315 | TCACACATGAAGG | 2574.21 |
| BBa_K1725316 | TCACACACGAAGG | 2352.63 |
| BBa_K1725317 | TCACACAGGAAAG | 2161.77 |
| BBa_K1725318 | TCACACAAGAGAG | 1969.19 |
| BBa_K1725319 | TCACACATGAGAG | 1506.8 |
| BBa_K1725320 | TCACACACGAGAG | 1500.04 |
| BBa_K1725321 | TCACACAGAAGGG | 1203.18 |
| BBa_K1725322 | TCACACAAAAGAG | 875.94 |
| BBa_K1725323 | TCACACACAAGAG | 875.94 |
| BBa_K1725324 | TCACACAGAAGAG | 875.94 |
| BBa_K1725325 | TCACACATAAGAG | 875.94 |
| BBa_K1725326 | TCACACAAGAAAG | 355.35 |
| BBa_K1725327 | TCACACAAAAAAG | 271.26 |
| BBa_K1725328 | TCACACACAAAAG | 271.26 |
| BBa_K1725329 | TCACACAGAAAAG | 271.26 |
| BBa_K1725330 | TCACACATAAAAG | 271.26 |
| BBa_K1725331 | TCACACATGAAAG | 271.26 |
| BBa_K1725332 | TCACACACGAAAG | 158.07 |
Randomised sequence locations are shown in red. Estimated strength corresponds to the Translation Initiation Rate values predicted by RBS library calculator for luxA gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]