Difference between revisions of "Part:BBa K515100:Experience"
 
(13 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<html>
 
<html>
−
<img src="https://static.igem.org/mediawiki/parts/0/03/Standard_curve.PNG" width=500px/>
 
 
</html>
 
</html>
 +
 
===Applications of BBa_K515100===
 
===Applications of BBa_K515100===
 +
*The enzymatic reactions of the IAM pathway produce indole-3 acetic acid (IAA) which is an important phytohormone.
 +
*Many Plant Growth Promoting Rhizobacteria produce IAA
 +
*Analogues of phytohormones like α-Naphthaleneacetic acid are usually found in rooting powders. Therefore, if we were to express IAA in bacteria in a controlled fashion we could increase root growth.
 +
*Increased root growth could help maintain top soil.
 +
*Evidence has shown that using IAA on cotton can increase yields.
  
 
===User Reviews===
 
===User Reviews===
Line 17: Line 22:
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 +
 +
 +
{|width='80%' style='border:1px solid gray'
 +
|-
 +
|width='10%'|
 +
<partinfo>BBa_K515100 AddReview 5</partinfo>
 +
<I>NUDT_CHINA 2015</I>
 +
|width='60%' valign='top'|
 +
 +
This part is the only one available with IAAM and IAAH , but unfortunately, we noticed that neither of the CDS of those two enzyme were submitted to the registry (they were only registered as BBa_K515000 and K515001). Thus, several improvements were made based on the part K515100 to provide an available version of IAAM and IAAH.
 +
 +
====Two pairs of enzymes that can PCR-prep the CDS of IaaM and IaaH====
 +
 +
For IAAM
 +
 +
F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’
 +
 +
R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’
 +
 +
 +
For IAAH
 +
 +
F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’
 +
 +
R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’
 +
 +
====Two new bio-bricks were designed based on this part using the primers above====
 +
 +
See BBa_K1789000 and BBa_K1789001
 +
 +
Both of those parts were sent to the registry.
 +
|};
 +
 +
 
<!-- DON'T DELETE --><partinfo>BBa_K515100 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K515100 EndReviews</partinfo>

Latest revision as of 16:27, 18 September 2015

Applications of BBa_K515100

  • The enzymatic reactions of the IAM pathway produce indole-3 acetic acid (IAA) which is an important phytohormone.
  • Many Plant Growth Promoting Rhizobacteria produce IAA
  • Analogues of phytohormones like α-Naphthaleneacetic acid are usually found in rooting powders. Therefore, if we were to express IAA in bacteria in a controlled fashion we could increase root growth.
  • Increased root growth could help maintain top soil.
  • Evidence has shown that using IAA on cotton can increase yields.

User Reviews

UNIQ61915f0277b4ba8b-partinfo-00000001-QINU


•••••

NUDT_CHINA 2015

This part is the only one available with IAAM and IAAH , but unfortunately, we noticed that neither of the CDS of those two enzyme were submitted to the registry (they were only registered as BBa_K515000 and K515001). Thus, several improvements were made based on the part K515100 to provide an available version of IAAM and IAAH.

Two pairs of enzymes that can PCR-prep the CDS of IaaM and IaaH

For IAAM

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’

R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’


For IAAH

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’

R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’

Two new bio-bricks were designed based on this part using the primers above

See BBa_K1789000 and BBa_K1789001

Both of those parts were sent to the registry.

;


UNIQ61915f0277b4ba8b-partinfo-00000003-QINU