Difference between revisions of "Part:BBa K1819002"
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==TAT signal==
 
==TAT signal==
  
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Twin-arginine translocation signal (TAT signal) can be used to protein secretion to the extracellular environment of ''Escherichia coli''.
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Twin-arginine translocation signal (TAT signal) can be used to protein secretion to the extracellular environment of ''Escherichia coli''.  
  
 
SacI restriction site was added at the 3’ flanking region of TAT signal to link it at the first amino acid residue of the protein to be exported.
 
SacI restriction site was added at the 3’ flanking region of TAT signal to link it at the first amino acid residue of the protein to be exported.
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<br>
 
<br>
 
</html>
 
</html>
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Please note this part was submitted in pSB1C3, the Registry's standard shipping backbone, according to submission requirements. However, pSB1C3  contains a SacI site and if you want to use this part we recommend to move the part into another plasmid backbone what can be easily done.
  
 
<partinfo>BBa_K1819002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1819002 SequenceAndFeatures</partinfo>

Revision as of 16:17, 18 September 2015

TAT signal

Twin-arginine translocation signal (TAT signal) can be used to protein secretion to the extracellular environment of Escherichia coli.

SacI restriction site was added at the 3’ flanking region of TAT signal to link it at the first amino acid residue of the protein to be exported.




Please note this part was submitted in pSB1C3, the Registry's standard shipping backbone, according to submission requirements. However, pSB1C3 contains a SacI site and if you want to use this part we recommend to move the part into another plasmid backbone what can be easily done.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]